Matrilin-2 Is Proteolytically Cleaved by ADAMTS-4 and ADAMTS-5
نویسندگان
چکیده
منابع مشابه
Matrilin-2 is proteolytically cleaved by ADAMTS-4 and ADAMTS-5.
Matrilin-2 is a widely distributed, oligomeric extracellular matrix protein that forms a filamentous network by binding to a variety of different extracellular matrix proteins. We found matrilin-2 proteolytic products in transfected cell lines in vitro and in mouse tissues in vivo. Two putative cleavage sites were identified in the unique domain of matrilin-2; the first site was located between...
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1Department of Rheumatology, Cardiff University and 2Connective Tissue Biology Laboratories, Cardiff School of Biosciences, Cardiff University, Cardiff, UK. Jan Bondeson, MD PhD; Shane Wainwright, PhD; Clare Hughes, PhD; Bruce Caterson PhD. This work has been supported by the Arthritis Research Campaign (UK), grants no. W0596, 13172 and 14570. Please address correspondence to: Prof. Bruce Cater...
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Little is known about the expression or role of ADAMTS-1, -4 and -5 and their endogenous inhibitor TIMP3 in the liver in physiological and pathological conditions. Their expression was, therefore, investigated in the hepatocellular carcinoma cell lines HepG2 and HuH-7 using qRT-PCR and western blotting, and their cellular localisation by immunocytochemistry. Cytokine treatments were used to ass...
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OBJECTIVE Recent published studies have shown that cartilage from ADAMTS-5-knockout mice, but not ADAMTS-4- or ADAMTS-1-knockout mice, is significantly protected from degradation. The present study was undertaken to evaluate the respective roles of these enzymes in human cartilage breakdown, using a small interfering RNA (siRNA) approach to assess the effects of inhibition of each enzyme in nor...
متن کاملAlpha2-macroglobulin is a novel substrate for ADAMTS-4 and ADAMTS-5 and represents an endogenous inhibitor of these enzymes.
Osteoarthritis is characterized by the loss of aggrecan and collagen from the cartilage extracellular matrix. The proteinases responsible for the breakdown of cartilage aggrecan include ADAMTS-4 (aggrecanase 1) and ADAMTS-5 (aggrecanase 2). Post-translational inhibition of ADAMTS-4/-5 activity may be important for maintaining normal homeostasis of aggrecan metabolism, and thus, any disruption t...
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ژورنال
عنوان ژورنال: Molecules
سال: 2014
ISSN: 1420-3049
DOI: 10.3390/molecules19068472